Sylvester is the enemy of Tweety Bird and Speedy Gonzales and the main antagonist of the Tweety and Speedy levels. In the Tweety levels, he chases Tweety, hoping to eat him, but gets defeated when Tweety hops into his birdcage and Granny slams the door on him. In the Speedy levels, he gets defeated when Speedy slams the door on him.
- Sylvester is the only antagonist of Looney Tunes Dash! to chase more than one playable character.